File S1 - Overexpression of Allene Oxide Cyclase Improves the Biosynthesis of Artemisinin in Artemisia annua L.

Contains the following files: Figure S1. Gus-staining of transgenic A. annua using the pCAMBIA1391Z empty vector. Figure S2. Analysis of AaAOC-overexpression transgenic A.annua by PCR. (a) PCR analysis using 35S forward primer and AaAOC reverse primer in AaAOC-overexpression transgenic A. annua. (b) PCR analysis using 35S forward primer and reverse primer of NPTII gene in AaAOC-overexpression transgenic A. annua. Figure S3. Chromatograms and mass spectrometry of JA and DHJA standards. (a) Chromatograms and mass spectrometry of JA. Peak1 is JA and the retention time is 13.399 min. (b) Chromatograms and mass spectrometry of DHJA. Peak2 is DHJA and the retention time is 13.456 min. Figure S4. The phenotypes of the transgenic A.annua and empty vector line. Figure S5. The synthetic pathway of artemisinin in A.annua. FPS, farnesyl diphosphate synthase; ADS, amorphadiene synthase; CYP, cytochrome P450 dependent hydroxylase (CYP71AV1); CPR, cytochrome P450 reductase; DBR2, double bond reductase 2; Aldh1, aldehyde dehydrogenase 1. Table S1. Primers used in this investigation. (DOC)