Epidermal fatty acid binding protein prevents chemical-induced skin tumorigenesis by inhibition of SOX2 expression in keratinocytes

E-FABP expression in keratinocytes increase interferons, in particualur IFNlamda, expression, which activate P53, a critical tumor suppessor, to inhibit or prevent chemical-induced skin tumorigenesis. Wild type(WT) mice and E-FABP deficient(EKO) mice were treated with DMBA/TPA according to procotol for 5 months. Skin tumors from WT and E-FABP-deficient mice (n=3/group) were collected and digested to single cells. Total RNA was extracted from 1 millions of tumor cells using RNeasy mini Kit and subjected to mouse mRNA gene expression by affymetrix microarray