Brn3a+ and FG+ RGC counts in Group 1 wholemounted retinae.

Immunohistochemically stained wholemounted retina, stained for Brn3a (red; Ai and Aii) and FG (gold; Aiii and Aiv; photographs taken in the same field, respectively), taken from Group 1a animals (intact optic nerve; Ai and Aiii) and Group 1b animals (21 days after ONC; Aii and Aiv). All images are representative of the 12 images taken per retina from 6 different animals (scale bar: 50 µm). In (B), the mean number of Brn3a+ and FG+ RGCs in a 1 mm2 region, from Group 1a and Group 1b rats, calculated as an composite average of 12 images, or an average of 4 images taken at 1.5 mm, 2.5 mm and 3.5 mm from the optic nerve head. Asterisks indicate significant difference at p