Within- and transgenerational stress legacy effects of ocean acidification on red abalone (Haliotis rufescens) growth and survival

Understanding the mechanisms by which individual organisms respond and populations adapt to global climate change is a critical challenge. The role of plasticity and acclimation, within and across generations, may be essential given the pace of change. We investigated plasticity across generations and life stages in response to ocean acidification (OA), which poses a growing threat to both wild populations and the sustainable aquaculture of shellfish. Most studies of OA on shellfish focus on acute effects, and less is known regarding the longer-term carryover effects that may manifest within or across generations. We assessed these longer-term effects in red abalone (Haliotis rufescens) using a multi-generational split-brood experiment. We spawned adults raised in ambient conditions to create offspring that we then exposed to high pCO2 (1,180 μatm; simulating OA) or low pCO2 (450 μatm; control or ambient conditions) during the first three months of life. We then allowed these animals to..., We ran a fully crossed factorial, split-brood experiment with a low pCO2 (mean condition: 450 matm, pHT 8.00, Waragonite = 2.08) treatment representing non-upwelling, current ocean conditions and a high pCO2 (mean condition: 1,180 matm, pHT 7.65, Waragonite = 0.91) treatment representing strong upwelling pH values observed along the California coast, as well as near-future persistent values predicted to become increasingly common with ocean acidification. We created F1 offspring from brood stock (F0) described below and carried out F1 and F2 exposure treatments during the parents’ (F1) larval and post-settlement phases (first three months of life; F1 early-life exposure; detailed in Swezey et al. 2020), the parents’ (F1) mature reproductive phase (for 14 months total prior to spawning; F1 adult exposure), and again during the offsprings’ (F2) larval and post-settlement phases (first three months; F2 early-life exposure) (Fig. 1). We performed experiments in a flow-through system used in..., , # Within- and transgenerational stress legacy effects of ocean acidification on red abalone (Haliotis rufescens) growth and survival

Data derived from experimental measurements during multiple stages of the experiment (F1 adult through F2 early life) analyzed in the categories outlined below. Each category has its own R notebook and corresponding data:

* F1 adult performance (growth in terms of length and weight)
* F1 adult fecundity (spawning success; gonad tissue thickness measured via ultrasound)
* F2 performance (survival at 10, 28, and 97 DPF; size at 10 and 97 DPF; Inorganic/Shell mass at 97 DPF)
* Data documenting seawater chemistry (temperature and pH/pCO2) throughout these phases of the experiment also included.

## Description of the data and file structure

**F1 Adult performance**
Length and Weight measurements analyzed for F1 adult abalone. Data taken in Fall of 2019, Fall of 2020, and Spring of 2021. Difference in length and weight from Fall 2019 to Fall 2020 u...