Temporal gene expression changes following EWSR1/FLI1 inhibition in EW-8 cells

Human Ewing sarcoma cell line EW-8 with different levels of EWSR/FLI1 using siRNA-EWSR1 were collected and profiled using single-cell RNA-seq. The single-cell RNA-sequencing data identified diverse reponses leading to the prediction of two rare cell identities in an unperturbed population (si-Control) with stem-like and dormant-like characterisitics. These newly discovered cell states may play important roles in undrstanding cancer heterogeneity, drug resistance and recurrence in Ewing sarcoma . Overall design: Three population of EW-8 cells were studied for their gene expression information in a single cell level (Unperturbed EW-8 or si-Control, Dormant 1 population or si-EWSR1-48h, Dormant 2 population or si-EWSR1-120h)