The expression profiling of the early stage of multiple system atrophy model mice

Global transcriptional analysis of the brain of multiple system atrophy model mice after synuclein induction by tamoxifen. Multiple system atrophy (MSA) is pathologically characterized by accumulation of phosphorylated α-synuclein in the oligodendrocytes. The pathophisiological mechinism under the early staige of disease pregression has been unknown. To clarify molecular alteration just after α-synuclein overexpression in the oligodendrocytes, we performed whole transcriptome analysis of the brain obtained from MSA model mice and control at 10 days after α-synuclein induction. Knock-in mice harboring human synuclein (Syn) gene with stop codon between loxp recognition sites were mated with mice expressing cre/estrogen receptor under the proteolipid protein (plp) promoter, which is an oligodendrocyte specific marker. Resulting mice (plp-Syn; Cre+/-) express human synuclein in the oligodedrocyte only after tamoxifen injection, and used as MSA model mice in this analysis. MSA model mice or control (plp-Syn; Cre-/-, n=3, respectively) were sacrified under deep anesthesia and brains were quickly removed. Right hemisphere of the brain from MSA model mice and control were subjected to transcriptome analysis or qRT-PCR.