Evolutionary assembly of the plant terrestrialization toolkit from protein domains

Land plants (embryophytes) came about in a momentous evolutionary singularity: plant terrestrialization. This event marks not only the conquest of land by plants but also the massive radiation of embryophytes into a diverse array of novel forms and functions. The unique suite of traits present in the earliest land plants is thought to have been ushered in by a burst genomic novelty. Here, we asked the question of how these bursts were possible. For this, we used phylostratigraphy to explore the (i) initial emergence and (ii) reshuffling of domains to give rise to hallmark environmental response genes of land plants. We pinpoint that a quarter of the embryophytic stress physiology is specific to the lineage, yet a significant portion of this novelty arises not de novo but from re-shuffling and recombining of pre-existing domains. Our data suggest that novel combinations of old genomic substrates shaped the plant terrestrialization toolkit, including hallmark processes in signaling, bioti..., , , # Orthogroup data used for: Evolutionary assembly of the plant terrestrialization toolkit from protein domains

## Description of the data and file structure

We have submitted all required data and information to recreate the main findings of our study. For ease, we have structured the detailed information on this by the main figures that are found in the manuscript. These are the Orthofinder-based results used for the study \"Evolutionary assembly of the plant terrestrialization toolkit from protein domains\". Each TSV file presents the direct output from the orthofinder.

## Sharing/Access information

Data was derived from the following sources:

(i) six species of chlorophyte algae, (ii) seven species of streptophyte algae, five bryophytes, and 14 tracheophytes. Orthogroups were built on protein data from five species of cyanobacteria were included as an outgroup: (HulseKemp et al. 2021), Arabidopsis thaliana (Lamesch et al. 2012), Arabidopsis lyrata (V2.1; Hu et al. 2011); Rawat et ...