Genetic interactions between REV3 or MMS2 and the MRX genes with respect to MMS sensitivity.

(A–C) Cell survival in a serial dilution assay. Overnight cell cultures were spotted on YPD or YPD containing DNA-damaging agents at the indicated concentration. The plates were incubated at 30°C for 2 days before being photographed. For UV treatment, the YPD plate was exposed to the indicated UV dose and incubated in the dark. All strains used are isogenic to BY4741. It should be noted that for each DNA-damaging agent, several concentrations/doses were examined and only one of the most appropriate concentration/dose is presented for each agent. (A) mre11 vs. rev3 or mms2; (B) rad50 vs. rev3 or mms2; (C) xrs2 vs. rev3 or mms2. (D,E) Cell survival in a liquid killing assay. These results are the average of three independent experiments with standard deviations indicated by error bars. (D) rad51 vs. rev3 or mms2; (E) mre11 vs. rev3 or mms2. All strains used are isogenic to BY4741.