RiboTag and bulk RNA sequencing using AAV-Control and AAV-shGpr151 infected sensory neurons.

To investigate the role of the Gpr151 mRNA and its interaction with ribosomal protein mRNA operon in regenerating neurons, we performed total RNA and RiboTag sequencing analysis using AAV-Control or AAV-shGpr151 infected sensory neurons using RiboTag mice, RPL22HA+/+;Advillin-Cre+. RiboTag sequencing data from cultured sensory neurons infected with AAV-Control and AAV-shGpr151. Preconditioning through sciatic nerve injury was conducted on mice before the neuron culture to activate their regenerative state.