scRNA-seq analysis (drop-seq) of pharmacological HCN channel block with ZD7288 on rat primary cortical neuronal stem cells

We analysed transcriptomic changes of single cell transcriptomes from primary rat cortical neuronal stem cells (NSC) after pharmacologically blocking HCN channels with ZD7288 compared to untreated NSC. This drop-seq scRNA-seq aproach showed accumulation of ZD7288 treated cells in G1 phase and confirmed our previous findings from a well-based scRNA-seq approach with cell cycle arrest in G1 after HCN channel block. scRNA-seq of two neuronal stem cell populations, one treated one non-treated