The LC-QTOF-MS/MS analysis of acid degradation products of Rifaximin, An Antibiotic

The present research aims to propose a simple and accurate technique for the
analysis of Rifaximin in the presence of its stress degradation products and analysis of
degradation products by LC-MS/MS analysis. Rifaximin was submitted to forced
degradation under acid hydrolysis condition as prescribed by the ICH.The active
Rifaximin was complementary provided by Hilton Pharma Pvt Ltd, and the extract was
prepared by firstly treated with HCl and heated about 4 to 8 hours. The filtrate was
collected and separated using dichloromethane followed by evaporation in rotary
evaporator to obtain a solid crude extract which was then stored under refrigeration at -
80 0 C. Liquid chromatography quadrupole time of flight mass spectrometry (LC-
QTOF-MS/MS) was utilized to identify products in the drug sample. The data
processing results revealed the presence of 9 products in the degraded sample of
Rifaximin. This data article contains the m/z [M+H + ] values, molecular formula,
retention times and the comprehensive list of m/z values detected during the LC-
QTOF- MS/MS analysis.