Regularity of Chondrocyte Phenotypic Changes Identified by Single-cell Sequencing in vitro

The specific mechanism of phenotypic change of chondrocytes in vitro remains unclear. We performed single-cell sequencing of P1,P3, and P6 cells in vitro to explore the mechanisms involved. Overall design: Chondrocytes were isolated from human auricular cartilage and cultured in vitro. Then, single cell sequencing was performed on 1,3, and 6 passage of cells.