Live imaging and functional characterization of the avian hypoblast

Images and movies related to the paper "Live imaging and functional characterization of the avian hypoblast redefines the mechanisms of primitive streak induction".

- Live imaging of the hypoblast of trasngenic quail embryos during embryonic axis initiation (Figure 1).
- Photoconversion of mEOS2 in the epiblast and the hypoblast (Figure 2a).
- Simultaneaous live imaging of the epiblast and the hypoblast in a 2-color chimera (Figure 2b-d).
- Live imaging of embryos in which a filter was intercalated between the epiblast and the hypoblast (Figure 2e-g)
- HCR-RNA-FISH against NODAL in animals of different timings (Figure 3a-b)
- FOXA2 and NODAL RNA labelling (Figure 3c-d)
- CER1 and NODAL RNAs labelling (Figure 3e)
- Staining made in embryos in which an Activin-coated bead was grafted (Figure 4)
- Live imaging and staining in embryos in which the hypoblast was fully ablated (Figure 4)
- Live imaging and staining in embryos in which a portion of posterior hypoblast was ablated (Figure 4)
- Live imaging and staining in embryos in which posterior hypoblast was grafted anteriorly (Figure 4)
- Staining in SB505124-treated embryos (Figure 4)
- Staining in embryos in which 2h-anterior hypoblast cells were grafted onto a 2h-host (Figure 5)
- Staining in embryos in which 7h-anterior hypoblast was grafted onto the posterior side of a 7h-host (Figure 5)
- Timelapse movies and staining of embryos in which 7h-posterior epiblast was grafted onto the anterior side of a 2h-host (Figure 5)
- Proof of feasability of hypoblast dissection at 7h (Supplementary Fig.1c)
- Confirmation of hypoblast motion with a chimera in which only the hypoblast expresses fluorescent reporters (Supplementary Fig.2)
- Images of hypoblast cells labelled with H2B-GFP at 7.5h and 12h, and inference of hypoblast cell area using Voronoi tessellation (Supplementary Fig.3h)
- Phospho-Myosin and ZO1 staining (Supplementary Fig.4)
- NODAL and GDF1 RNAs labelling (Supplementary Fig.5)
- Timelapse movies of chimeras in which posterior and anterior hypoblast is labelled in 2 different colors (Supplementary Fig.6b)
- Backtracking of FOXA2-low/NODAL-high region (Supplementary Fig.6c)
- Live imaging and staining of chicken embryos in which the hypoblast was fully ablated (Supplementary Fig.7c)
- Live imaging and staining of embryos in which hypoblast was ablated and grafter back (Supplementary Fig.7c)
- Live imaging and staining of embryos in which an Activin-A coated (or control) bead has been grafted posteriorly after hypoblast removal (Supplementary Fig.8a)
- Live imaging and staining of embryos on which a control bead was deposited (Supplementary Fig.8b)
- Staining in embryos treated from 2 to 6h with SB-505124 (Supplementary Fig.8c)
- Staining of embryos in posterior hypoblast was grafted posteriorly at 2h (Supplementary Fig.9c)
- Timelapse movies and staining of embryos in which 7h-anterior epiblast was grafted onto the anterior side of a 2h-host (Supplementary Fig.9d)
- Staining of embryos in which 7h-anterior hypoblast was grafted onto the anterior side of a 2h-host (Supplementary Fig.10b)