_A_LA_myc-GC-MS.output.raw

pISA-tree file path:    _p_SUSPHIRE\_I_T33_MonoterpenoidsFungi\_S_P5_SxF_lav_ac\_A_LA_myc-GC-MS\output\raw

Title:    Agroinfiltration of final constructs for copper-inducible pathway in Nicotiana benthamiana leaves
Description:    Two constructs were made containing the three genes of the moth pheromone pathway under the regulation of synthetic promoters made in T11, nptII selection marker and with/without the gRNA1 for activation. In parallel, another construct containing the copper-inducible dCas9EV system with/without the guide was made in pLX vector so it could be co-transformed in the same agrobacterium colony with the moth pheromone pathway. Such Agrobacterium containing both plasmids (guided/nonguided pathway + nonguided/guided copper-inducible dCas9EV) was agroinfiltrated before stable transformation in Nicotiana benthamiana to check the expression of the genes.