Cross-dataset concordance and divergence of ALS and spaceflight transcriptomic signatures: data and code

Per-contrast DESeq2 differential expression results for 37 contrasts from nine public datasets (ALS post-mortem spinal cord and motor cortex from the NYGC Consortium; ISS-flown cortical and dopaminergic organoids with and without microglia; simulated GCR irradiated organoids; ground-based microgravity analogs in human motor neurons; mouse spaceflight brain regions from the RRRM-2 rodent research missions; OSD-202 mouse stressor dissections). Includes the curated gene set database (155 mechanism/disease sets plus KEGG-ALS-unique, Tier-3 subtype markers, Tier-4 cell-type markers, and 100 negative-control sets), fgseaSimple and fgseaMultilevel (seed 42) enrichment outputs, the primary analysis pipeline (empirical category anchoring, weighted concordance score, RBP knockdown and subtype analyses), an independent gseapy revalidation of all 37 contrasts, and gene-level Spearman correlations with permutation p-values. All manuscript numbers trace to results/canonical_outputs/numbers_manifest.json.