Figure S3_G Factor ROI data

2 Biological Replicates. See Methods_S1 from our manuscript for details on sample preparation. Refer to Platemap for Well ID. Intensity data acquired with 3 different configuration on the INO -FHS. ROIs were selected and applied uniformly to images acquired with each configuration. \"Well ID given at the beginning of Filename. ie. C2 is in row C column 2. \"FOV\" indicates the field of view. \"CFP\" indicates only donor (mCerulean3) excitation, \"YFP\" indicates only acceptor (Venus) excitation and \"FRET\" indicates both channels excited simultaneously. \"segmentmap\" is a copy of the ROIs selected and saved. Use as example to run our G-factor code on Github. Final analyzed data is displayed in Figure S3.