Impact of UCP1 and FGF21 for the development of diet-induced obesity at standard housing temperatures

Global RNA sequencing analysis of brown fat (BAT), inguinal white fat (iWAT), liver and muscle (quadriceps) of high-fat diet fed WT, FGF21 KO, UCP1 KO and UCP1/FGF21 double KO mice. Overall design: The experiment was performed in homozygous, male WT, FGF21 KO, UCP1 KO and UCP1/FGF12 double KO (dKO) mice. All mice were bred, born and weaned at 30°C. At the age of 10 to 12 weeks, mice were transferred from 30°C to room temperature and chow diet was changed to high-fat diet for 3 weeks. At the end of the experiment, mice were euthanized after 2-3 hours food withdrawal, followed by serum and tissue sample collection.