Two parameters are required to observe a strong ~ 75kDa streptavidin-binding band for growth medium supplemented with L-methionine. (Note that AHA was not present for any of the samples.) When combined, the alkylation with IAA and modification with DBCO-PEG4-biotin can generate false positive results for medium alone and the medium/secretome fraction of cultured cells. This is demonstrated by streptavidin binding to material of ~75kDa (Fig 3).
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https://figshare.com/articles/dataset/Two_parameters_are_required_to_observe_a_strong_75kDa_streptavidin-binding_band_for_growth_medium_supplemented_with_L-methionine_Note_that_AHA_was_not_present_for_any_of_the_samples_When_combined_the_alkylation_with_IAA_and_modification_wit/29913920
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Two parameters are required to observe a strong ~ 75kDa streptavidin-binding band for growth medium supplemented with L-methionine. (Note that AHA was not present for any of the samples.) When combined, the alkylation with IAA and modification with DBCO-PEG4-biotin can generate false positive results for medium alone and the medium/secretome fraction of cultured cells. This is demonstrated by streptavidin binding to material of ~75kDa (Fig 3).
创建时间：
2025-08-14
