Sequencing of the whole transcriptome of papillary thyroid cancer and lymph node metastases

This data was gathered from three patients who had papillary thyroid carcinoma with paired lymph node metastases. The goal of this study was to determine critical genes and pathway that influence the occurrence of lymph node metastasis in PTC patients. A total of 6 samples were analyzed, resulting in an average of 10.16 GB of data per sample. 1,967 new genes were predicted; 19,430 genes were detected as expressed, of which 17,890 were known genes and 1,540 were predicted new genes; 27,495 new transcripts were found, of which 15,846 belonged to known genes. There were a total of 27,495 novel transcripts discovered, with 15,846 belonging to new variable splice isoforms of known protein-coding genes, 1,967 to transcripts of new protein-coding genes, and 9,682 to long-stranded non-coding RNAs. Comparative gene expression profiling analysis of RNA-seq data for primary tumor and lymph node metastasis of papillary thyroid cancer (PTC).