Figure S2 - Phospholipase C-Related Catalytically Inactive Protein (PRIP) Regulates Lipolysis in Adipose Tissue by Modulating the Phosphorylation of Hormone-Sensitive Lipase

Accumulation of PRIP2 and PP2A at the peripheries of lipid droplets in COS7 cells after adrenaline stimulation. COS7 cells were transfected with GFP-tagged PRIP2 and myc-tagged PP2A. The cells were cultured overnight with oleic acid and then stimulated with (+) or without (–) adrenaline (5 µM) for 5 min. PP2A was detected with an anti-PP2A antibody followed by anti-mouse IgG labeled with Alexa Fluor 405. The cells were visualized by confocal microscopy. Two sets of representative images are shown. Boxed areas of the left image (scale bar: 10 µm) are enlarged in the four right images (scale bar: 5 µm). Two independent experiments were performed, and similar images were obtained. The arrowhead in the GFP-PRIP2 and PP2A images indicates an accumulation of each signal at the lipid-droplet periphery.
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