Next Generation Sequencing Quantitative Analysis of altered expression of genes in DHCR24 overexpressing bladder cancer cells

Bladder cancer (BCa) is one of the most commonly diagnosed urinary malignancies with an estimated yearly 550,000 new cases worldwide. Previous study revealed that DHCR24 was a crucial oncogene that widely involved in tumor initiation and progression. Yet the biological role and underlying regulatory mechanism of DHCR24 in lymph node (LN) metastasis of bladder cancer (BCa) remain unclear. Our study aims to explore the target genes of DHCR24 and the underlying mechanism in the LN metastasis of BCa. To detect the precise mechanism of DHCR24 in LN metastasis of BCa, the total RNA of UM-UC-3 cells transfected with Vector plasmids or DHCR24-overexpressing plasmids was extracted and the next generation sequencing was conducted to explore the expressing profile difference between Vector and overexpression group. The result was obtained from three independent experiments.