Transcriptome profiling of miR-541 kd mouse cortical cells

Using an in vitro model of mouse corticogenesis from mouse embryonic stem cells (ESCs), we investigated the gene expression changes exerted by the inactivation of the Eutherian-specific microRNA miR-541. The microRNA mmu-miR-541-5p was inhibited by the transfection of an antago-miR into ESC-progenitors at 12 days of in vitro differentiation (DIV12) and and the cell transcriptome of transfected cells was compared to the transcriptome of control-transfected cells 5 days after transfection (DIV17). The comparison between control and miR-541 kd cells highlighted classes of differentially expressed genes related to axon elongation and neural development GO terms. Transcriptome datasets of mouse embryonic cortical cells transfected with antago-miR against mmu-miR-541-5p or with control antago-miR