Supplemental Material for Benbow, Jermiin, and Doohan, 2019

Supplementary file 1 is a fasta file multiple sequence alignment (created with Clustal Omega) of wheat serpin proteins. Supplementary file 2 is a fasta file multiple sequence alignment (created with Clustal Omega) when uninformative sites masked (removed). Quality scores for the original alignment were generated with AliStat, and sites in the alignment that had a completemess score of < 0.4 were removed.Supplementary file 3 is a fasta file multiple sequence alignment (created with Clustal Omega) with imcomplete sites removed (as per supplementary file 2), and sequences removed to avoid the alignment containing any pairs of sequences that contain no overlap. This is final alignment that was used for creation of the wheat serpin phylogenetic tree.Supplement figures and tables file contains:Tables S1 and S2 - tables that give details of the publicly available RNAseq data that was used to profile the expression of the wheat serpin genes.Figure S1 - A heatmap showing differential expression of wheat serpins in the grain at three time points post inoculation with Fusarium graminearum in two wheat cultivars.Figure S2 - A phylogenetic tree of a sub-clade of wheat serpins that contains five Fusarium graminearum reponsive serpin genes.

补充文件1为小麦丝氨酸蛋白酶的多个序列比对fasta文件（由Clustal Omega创建）。补充文件2为在屏蔽（去除）非信息位点后的fasta文件多个序列比对（由Clustal Omega创建）。原始比对的质量评分由AliStat生成，且在比对中，完整性评分小于0.4的位点已被去除。补充文件3为fasta文件多个序列比对（由Clustal Omega创建），其中已去除不完整位点（参照补充文件2），并移除了为避免比对中包含任何无重叠序列对的序列。此为最终比对文件，用于构建小麦丝氨酸蛋白酶的进化树。补充图表和表格文件包含：表格S1和S2 - 提供了用于分析小麦丝氨酸蛋白酶基因表达谱的公开RNAseq数据的详细信息的表格。图S1 - 展示在接种Fusarium graminearum后三个时间点，两种小麦品种中小麦丝氨酸蛋白酶在籽粒中的差异表达的heatmap。图S2 - 显示包含五个对Fusarium graminearum响应的丝氨酸蛋白酶基因的亚系的 wheat serpins的进化树。