Effect of channel flow rate on Jurkat and HeyA8 cell separation.

Numbers presented represent the averages of the trails conducted.
At fast flow rate, the hydrodynamic force was dominant and pushed stiff cells migrated to soft outlet which resulted in the lowest enrichment. At slow flow rate, cells were stuck to the ridges and occluded the channel which resulted in the lowest throughput. We determined channel flow rate at provided the best separation result. The cell retention is defined by calculating the ratio of cells collected at the outlet and total number of cells injected in the inlet. For example, the stiff outlet retention is . The total retention is the sum of cells collected at the two outlets.