MAC Sevo N2O Data Plos One.xlsx

Animals were initially anesthetized with SEVO in oxygen (O2)
and exposed to an electrical current as supramaximal noxious stimulus in order
to determine MACSEVO (in duplicates). Thereafter, 70% N2O
was added to the inspired gas and the MAC re-determined in the presence of N2O
(MACSN). A subgroup of sheep were anesthetized a second time with
SEVO/N2O for re-determination of MACSN, after which
atipamezole (0.2 mg/kg, IV) was administered for MACSNA determinations. Sheep were anesthetized a third time, initially with only
SEVO/O2 to re-determine MACSEVO, after which atipamezole
(0.2 mg/kg, IV) was administered for determination of MACSA.