RNA-seq data on embryogenic callus regeneration

Regeneration of plant somatic cells is a prerequisite for their biological breeding. Identification of key genes controlling embryogenic callus (EC) differentiation and investigation of the genetic mechanism of cell fate determination are important for promoting plant variety improvement. Here, we used the somatic regeneration extreme maize inbred lines KN5585 and its peroxidase gene mutants Zmprx19 as study materials. RNA-seq technology was employed to sequence and conduct a comparative transcriptome analysis of their ECs at each of the four differentiation stages. The findings of this study provide a foundation for the identification of key genes involved in somatic regeneration and for further analysis of the molecular mechanisms underlying the regaining of totipotency in plant cells.