Affinities of peptides to MHC-II alleles.

Note: peptides marked in bold are positive controls included in the assay; HA306–318 is a promiscuous HLA-DR binder whereas hypothetical protein 239–253 has been shown to bind most HLA-DR alleles and the HLA-DP allele. Peptide showing affinities above 5000 nM are categorized as non-binders (NB).aPeptide binding affinity (KD) in nM.bPeptide reactivity blocked by anti-HLA-II subtype antibody in the responding donor number (See Fig. 2 and Table 5).cThe HLA-II subtype assayed for peptide binding was expressed by donor.dNo cells available for HLA-II typing.