Comparative RNA-seq analysis in the basal nodes of nitrogen-stressed hexaploid wheat Tad17 triple knock-out mutant (TILLING) and WT segregant plants

The aim of this study was to investigate the role of strigolactones (SLs) in controlling tillering and their involvement as a signal for nitrogen status. RNA-seq analysis was performed on 18-day-old hydroponically grown Tad17 triple knock-out TILLING mutant plants (SL-deficient mutant) and WT-segregant plants, 8 days after introducing them to nitrogen limiting conditions. The experiment consisted of two different treatments: High Nitrogen (10 mM N) and Low Nitrogen (0.1 mM N). Each treatment included six biological replicates. Total RNA was extracted from pooled samples of four basal node sections per biological replicate. In this study, the basal node was defined as the 0.5 cm section from the base of the main shoot, comprising the apical meristem, lateral buds, leaf meristems, etc.