Subtype-selective agonists of COI1-JAZ co-receptors screened from the stereochemical-isomers of jasmonate mimic

We report the development of subtype selective ligands into plant hormone jasmonate co-receptor COI1-JAZ. Plant hormone jasmonoyl isoleucine and the structural mimic natural product coronatine has non-selective affinity with 13 species of COI1-JAZ co-receptors in Arabidopsis thaliana to cause bath of bacterial defense and growth inhibition. Some subtype selective ligands were developed by our chemical screening and activated the part of whole jasmonate signaling. To examine the mode-of-action of the subtype selective ligand, gene expression profiles of WT and jaz9 mutant treated with mock or with coronatine (2) and subtype selective ligands (2e and 12) was performed using the RNA-seq. The subtype selective ligand induced partial activation of jasmonate signaling to cause defense responses against bacterial pathogen without growth inhibition. Overall design: Gene expression by the treatment of 1 µM of natural product coronatine (2) and the derivatives (2e and 12) for 8 h in A. thaliana WT seedlings. Gene expression by the treatment of 1 µM of natural product coronatine (2) and the derivatives (2e and 12) for 8 h in A. thaliana jaz9 mutant seedlings