Serial femtosecond X-ray structure of a fluorescence optimized bathy phytochrome PAiRFP2 derived from wild-type Agp2 in I2 intermediate state.

Serial femtosecond X-ray structure of a fluorescence optimized bathy phytochrome PAiRFP2 derived from wild-type Agp2 in I2 intermediate state. Descriptor: 3-[(2Z)-2-({3-(2-carboxyethyl)-5-[(E)-(4-ethenyl-3-methyl-5-oxo-1,5-dihydro-2H-pyrrol-2-ylidene)methyl]-4-methyl-1H-pyrrol-2-yl}methylidene)-5-{(Z)-[(3E,4S)-3-ethylidene-4-methyl-5-oxopyrrolidin-2-ylidene]methyl}-4-methyl-2H-pyrrol-3-yl]propanoic acid, CHLORIDE ION, DI(HYDROXYETHYL)ETHER, ... Authors: Sauthof, L, Schmidt, A, Szczepek, M, Brewster, A.S, Kern, J.F, Scheerer, P. Deposit date: 2023-12-21 Release date: 2025-05-14 Last modified: 2025-06-11 Method: X-RAY DIFFRACTION (2.43 Å) Cite: Serial-femtosecond crystallography reveals how a phytochrome variant couples chromophore and protein structural changes. Sci Adv, 11, 2025源自野生型Agp2的荧光优化型深海光敏色素PAiRFP2在I2中间态下的串行飞秒X射线晶体结构。描述项：3-[(2Z)-2-({3-(2-羧乙基)-5-[(E)-(4-乙烯基-3-甲基-5-氧代-1,5-二氢-2H-吡咯-2-亚基)甲基]-4-甲基-1H-吡咯-2-基}亚甲基)-5-{(Z)-[(3E,4S)-3-亚乙基-4-甲基-5-氧代吡咯烷-2-亚基]甲基}-4-甲基-2H-吡咯-3-基]丙酸、氯离子、二(羟乙基)醚…… 作者：Sauthof L、Schmidt A、Szczepek M、Brewster A.S、Kern J.F、Scheerer P。提交日期：2023-12-21；发布日期：2025-05-14；最后修改日期：2025-06-11。方法：X射线衍射（2.43 Å）。引用文献：《串行飞秒晶体学揭示光敏色素变体如何耦合生色团与蛋白质结构变化》，发表于《Science Advances》，2025年，第11卷。