Unbiased genomic analysis of murine lung cancer cell lines. Unbiased genomic analysis of murine lung cancer cell lines

To uncover the gene expression alterations that occur during lung cancer progression, we interrogated the gene expression state of tumor cell lines generated from different stages of malignant progression. We initiated tumors in KrasLSL-G12D/+;p53flox/flox (KP) mice. Cancer cells were isolated from individual primary tumors and metastases Overall design: This study includes 4 samples: 2 non-metastatic primary tumors, 2 metastasis in lymphnode. total RNA was isolated and prepared for sequencing using the Illumina TruSeq RNA kit to generate 100bp paired end reads. Reads were aligned to mm9.