ATAC-seq of HFF exposed to salt and heat stress

Primary human foreskin fibroblasts (HFF) were exposed to either salt stress (80mM KCl) or heat stress (44ºC). ATAC-seq libraries were prepared starting with 50,000 cells per condition following the protocol described by Buenrostro et al., Nature Methods 2013 Overall design: HFF were exposed either salt stress (80mM KCl) or heat stress (44ºC) for 1 or 2h. ATAC-seq samples were prepared at the indicated times of infection. Two independent biological replicates were analysed for salt and heat stress and four replicates for untreated cells.