Single cell RNA sequencing analysis of P63+ lung progenitor cells isolated from IPF patients

To gain molecular insights into the identity composition of the cells selectively grown in the culture system, we performed single-cell sequencing on the primary cell. Unsupervised clustering of the cells uncovered 3 distinct subpopulations:C1, C2 and C3. Altogether the data indicated that the current cell isolation and expansion system could efficiently enrich P63+ lung progenitor cells from IPF patients. Overall design: After removing feeder cells through gradient digestion, the primary cells isolated by the pharmaceutical-grade cell cloning culture system were digested to prepare a single-cell suspension. Single cells were captured and barcoded in 10× Chromium Controller (10× Genomics).