Global gene expression profile of anti-CD3/CD28 or PMA/ionomycin stimulated CD4+ T cell, wild type and Mapk1-KO CD4+ T cells, and empty vector and sh-Zfp831 infected CD4+ T cells

Several factors promote Tfh cell differentiation. In order to understand how they promotes the expression of Tfh signature genes, we conducted transcriptional analysis. Naive CD4+ T cells were sorted from wild type or Mapk1-KO mice and activated under various conditions. Under specified condition, empty vector or pMKO.1-sh-Zfp831 were viral transfected into cells. Three days later, the total RNA was extracted with Trizol (Life Technologies) according to manufacturer’s instructions. RNA-seq library was constructed and sequenced with BGI500 platform by BGI Genomics and successfully mapped to mouse genome. Reads counts were normalized based on RPKM/FPKM, fold changes were calculated for all possible comparisons.