Failure to recover Pseudomonas fluorescens D7 supports claims of ineffectiveness as biocontrol agent of Bromus tectorum

The biocontrol agent Pseudomonas fluorescens D7 is touted to control Bromus tectorum, yet in-field experimental results show limited efficacy. To better understand this lack of control, soil samples from an active cheatgrass invasion treated with P. fluorescens D7 were collected and subjected to high-throughput sequencing. No reads assigned to P. fluorescens D7 were found in our sequence data, indicating that the single application was insufficient for establishment.