Kinetic Activity Profiling of Galactose Oxidase Mutants for Biocatalytic Secondary Alcohol Oxidation

a curated dataset of kinetic colorimetric activity measurements of galactose oxidase variants for biocatalytic oxidation of a secondary alcohol, 1-phenylbutan-1-ol. Using the engineered galactose oxidase variant, GOh1052, as the backbone, site saturation mutagenesis libraries covering 352 residue positions were constructed, yielding 6,686 unique single mutants. These mutants were subsequently screened via a colorimetric assay to capture their kinetic activity profiles over a 16-hour reaction period.