OMAP-5 Organ Mapping Antibody Panel (OMAP) for Multiplexed Antibody-Based Imaging of Human Liver with SIMS, v1.4

OMAP-5 was designed for Secondary Ionization Mass Spectrometry (SIMS) imaging of fresh frozen human liver samples<br>(Tian et al. 2022). The panel contains 20 antibodies and a cationic nucleic acid intercalator that contains<br>natural-abundance iridium. An antibody directed against Na/K ATPase was used for cell segmentation and image<br>alignment. This OMAP provides spatial context for the anatomical structures, metabolic zonation, and cell types<br>present in the ASCT+B liver table v1.0 (Masci, Kendall, and Suzuki 2021). This OMAP does not yet contain antibodies<br>for NK cells, dendritic cells, portal fibroblasts, MAIT cells, gamma delta T cells, and hepatic oval cells. Antigens<br>were detected using lanthanide tagged primary antibodies where the metal tags were detected by specific mass channels<br>using C60 SIMS. Details on the antibody staining method can be found here (Tian 2022). Image registration and cell<br>segmentation were performed using Deepcell. Cell segmentation was performed using nuclear and membrane marker<br>channels. Integrated protein expression was used to determine thresholds for cellular classification. An antibody<br>directed against EGFR was originally included in OMAP-5 v1.0 and v1.1, but was removed due to inconsistent labeling. A<br>representative dataset using this OMAP can be found here: (Tian and Tarolli 2023).<br><br>**Bibliography:**<br><br>* Masci, Anna Maria, Tim Kendall, and Ayako Suzuki. 2021. “Liver (v1.0) Graph Data, v1.0.” https://lod.humanatlas.io/asct-b/liver/v1.0/.<br>* Tian, Hua. 2022. “Liver Tissue Staining with Multiple Lanthanides-Tagged Antibodies,” February. https://www.protocols.io/view/liver-tissue-staining-with-multiple-lanthanides-ta-b5qmq5u6.<br>* Tian, Hua, Presha Rajbhandari, Jay Tarolli, Aubrianna M. Decker, Taruna V. Neelakantan, Tina Angerer, Fereshteh Zandkarimi, et al. 2022. “Multi-Modal Mass Spectrometry Imaging Reveals Single-Cell Metabolic States in Mammalian Liver.” bioRxiv. https://doi.org/10.1101/2022.09.26.508878.<br>* Tian, Hua, and Jay Tarolli. 2023. “Multiplexed Staining Dataset - OMAP 5 - Liver-Lanthanides-Conjugated Antibodies and C60-Secondary Ion Mass Spectrometry Imaging.” Zenodo. https://zenodo.org/records/8084259.