Profiling Kupffer cells with different origins in NASH

Kupffer cells (KCs) self-renew by local proliferation in the adult independently from monocytes (Mos). However, how they maintain during chronic metabolic disorders such as non-alcoholic steatohepatitis (NASH) remains ill-defined. We characterized KCs during NASH and observed diversity in the KC pool, with a significant fraction of monocyte-derived KCs (MoKCs). Here, we aim to uncover the transcriptional landscapes of KCs subsets found in a mouse model of methionine and choline deficient (MCD)-diet induced non-alcoholic steatohepatitis (NASH). Overall design: Wild-type mice were fed a MCD diet for 4 weeks and Kupffer cell subsets were sorted by flow cytometry. EmKCs were CLEC2+ TIMD4+ while MoKCs were CLEC2+ TIMD4-.