Neu5Gc intake increases the expression of colorectal cancer-related genes in the APCMin+ mouse

Clinical cohort data suggest that high consumption of red meat containing N-glycolylneuraminic acid (Neu5Gc) may increase colorectal prevalence, therefore, we investigated the effect of high Neu5Gc intake on colon cancer using the APCMin/+ mouse model . Ten APCMin/+ male mice were divided into two groups and fed normal (control group: 0.0507 mg per mouse per day) and high concentrations (treatment group: 1.014 mg per mouse per day) of Neu5Gc to mimic Normal and excessive consumption of red meat in humans, respectively. After two weeks of feeding, colorectal and liver were collected for histopathological diagnosis and transcriptome analysis. Eight-week-old male APCMin/+ mice were divided into two groups, 6 mice in the control group and 4 mice in the treatment group. The control group drank low-concentration Neu5Gc water (0.0127mg/ml), and the treatment group drank high-concentration Neu5Gc water (0.2535mg/ml) for 14 consecutive days. Intestinal and liver tissues were collected after sacrifice, followed by histopathological diagnosis, intestinal and liver transcriptome analysis.