Analysis of intestinal crypt, villus, and polyp cells from WT and Vil1-Grem1 with or without anti-Grem1 antibody treatment by scRNA-seq

We aimed to examine changes in cellular composition of intestinal crypts, villi, and polyps from wild-type, Grem1-overexpressing (Vil1-Grem1) and anti-Grem1 antibody-treated (30 mg/kg subcutaneously weekly from the age of 6-weeks old for 10 weeks) Vil1-Grem1 mice by scRNA-seq. The proximal small bowel was dissected out the muscle layer was scraped off mechanically with a coverslip and discarded. The villus compartment and crypt compartment were separated mechanically and digested into a single cell suspension and processed using a 10X Genomics Chromium Single Cell 3' v3 kit and Chromium Instrument.