Flux predictions in R. toruloides batch cultivations on three different carbon substrates–glucose (G), xylose (X) and acetate (A) at exponential growth (exp) and nitrogen limitation (Nlim) phases.

Fluxes are calculated using random sampling of the solution space with 2000 iterations (mmol/gDCW/h) on R. toruloides enzyme-constrained genome-scale models. Fluxes represent median values and are normalized by dividing flux with specific substrate uptake rate (representing % of carbon distribution). Fluxes are represented in non-ec model (base GEM) annotation by merging forward and reverse fluxes created by the GECKO formalism. Flux variability is SD divided by the flux value, multiplied by 100. Flux changes were compared using log2 fold change (log2FC). (XLSX)