Transcriptome analysis after ectopically expressing REV in SAM epidermis

We ectopically expressed REV in epidermis in ap1 cal background by using AtML1 promoter driven inducible REV(GtoG)-2Venus for RNA-Seq studies. Expression of this transgenes in ap1 cal background gives phenotype similar to their expression in wild-type (WT) backgrounds. For pML1>>REV(GtoG)-2Venus , we sorted the epidermal cells using FACS, after their induction of 6 hours or 16 hours. As a control we collected the epidermal cells after 6 or 16hr induction of pML1::GR-LHG4 in pML1::BFPer background. Q-PCR analysis on extracted RNA showed the successful sorting and regulation of known target genes of REV. 2 different transgenic lines (pML1>>REVgto-2Venus, and pML1::GR-LHG4, pML1::BFPer) were used in ap1cal background. 2 time points (6hr and 16hr) were used for each transgenic line. 3-4 biological replicates were used for each time point. cells were collcted through FACS, and used for RNA-Seq analysis