Genomic comparison of Brucella 2308 and O. anthropi LMG3331 ORFs involved in the synthesis of surface exposed or OM PAMPs.

1Although acting in the O-PS biosynthetic pathway, these proteins are involved in the steps previous to O-sugar polymerization and, therefore, the corresponding sugars belong structurally to the core oligosaccharide.2These bacteria differ in O-PS sugar structure and have unrelated glycosyltransferases and O-PS export systems. Accordingly, only ORFs assigned to the core and lipid A are presented in the Table. Proteins known to be involved in ancillary pathways of Brucella core oligosaccharide (Pgm, ManBcore and ManCcore) are highly conserved in O. anthropi.3Only ORFs attributed in the annotations in databases to the lipid A modification systems [83] are included.4This ORF, although not annoted in the B.abortus 2308 genome, was identified by using Oant_1613 as a query in the TBLASTN program (NCBI).5For lipoproteins, lipoprotein annotations were obtained from both genomes and verified with the lipoP (http://www.cbs.dtu.dk/services/LipoP/) in those cases in which there were discrepancies between the two annotations.