16S metagenomic sequences of biofilm forming K. pneumoniae

The study evaluated an RT-PCR panel using mrkD, pgaC and wcaJ genes to screen for biofilm-forming K. pneumoniae from ETA/BAL specimens. Representative culture-negative-PCR-positive samples were subjected to metagenomics which identified K. pneumoniae reads in these samples confirming the specificity of RT-PCR. The result of this study highlights that the rapid detection of K. pneumoniae biofilms based on the RT-PCR results coupled with clinical conditions would be appropriate to treat emerging infections or to prevent re-infections in the clinical settings.