DNA damage in response to Aza as measured with the comet assay YUMAC.

Melanoma cells were untreated or treated with Aza for 2 days, harvested after one-day recovery in standard growth medium, and subjected to the Comet assay. Cells were examined with fluorescence microscope, photographed, and analyzed with CASP software (http://casp.sourceforge.net). The percentage of the DNA tail area was divided to total DNA area for each cell, and percentage of DNA tail length divided to total DNA length was counted. The data represent averages from 100–120 cells±SE; YURIF DNA damage response is a representative of two biological replicates with similar results. Treatment with hydrogen peroxide (100 µM) for 10 minutes on ice was used as positive control.