Plant sample from SC-CO2 garlic extract treated Triticum aestivum

The biostimulant phytochemicals as alternatives to synthetic chemicals are gaining ground in sustainable agricultural production nowadays. The medicinal herb, garlic (Allium sativum) has a spectacular therapeutic reputation due to its antimicrobial properties. The effectiveness of supercritical carbon dioxide (SC-CO2) extraction of A. sativum could help preserve bioactive compounds and be used as a biostimulant agent. The SC-CO2 garlic was formulated in liposomes and used as a nanoscale drug delivery system to reach better efficiency of penetration and translocation. The SC-CO2 garlic extracts were used in Triticum aestivum time-course experiments to monitor conditioning effects such as improving crop quality and priming its defense responses against different pathogens. Fresh leaves were collected after SC-CO2 garlic exposure at 15 min, 24, and 48 hours for QuantSeq 3′ mRNA sequencing at Illumina NextSeq 550 platform

生物刺激剂类植物化学物质作为合成化学品的替代品，在可持续农业生产的领域中正日益受到重视。药用草本植物大蒜（Allium sativum）凭借其抗菌特性而享有卓越的药用声誉。采用超临界二氧化碳（SC-CO2）提取法对A. sativum进行处理，有助于保留生物活性成分，并可作为生物刺激剂使用。将SC-CO2大蒜制成脂质体，用作纳米级药物递送系统，以提高渗透和迁移效率。在小麦（Triticum aestivum）的时程实验中，利用SC-CO2大蒜提取物以监测调节效果，如提升作物品质及其对多种病原体的防御反应的启动作用。在SC-CO2大蒜暴露15分钟、24小时和48小时后收集新鲜叶片，以在Illumina NextSeq 550平台上进行QuantSeq 3′ mRNA测序。