Genome-wide study of the role of the histone demethylase dLsd1 in oogenesis

The histone demethylase LSD1 is a key chromatin regulator that plays a crucial role in development. It has been shown to act both as a co-repressor and a co-activator of genes expression and to affect the expression of many genes important for development. In Drosophila, dLsd1 is essential for oogenesis; however, our current knowledge of dLsd1 function is insufficient to explain its precise role in the ovary. To better define the role of dLsd1 in oogenesis, we performed genome-wide analysis of dLsd1 binding in the ovary by Chromatin Immunoprecipitation followed by sequencing (ChIP-Seq). Overall design: ChIP-seq profiles of dLsd1 binding in young wild type (w1118) ovaries were generated by deep sequencing in triplicate. The ChIP-seq antibody was generated in the Dyson team in Boston [PMID 17462898]