Overview of wtf alleles and their phenotypes in this study.

Columns 1–2 describe the allele names and their construction. Columns 3–4 describe the toxicity of the poison and antidote proteins encoded by the corresponding allele. Column 5 notes if the mutant antidote can rescue the corresponding poison allele. Column 6–7 note if the specific antidote allele can rescue the wild-type poison (Column 6), or if the specific poison can be rescued by the wild-type antidote (Column 7). Column 8 notes if the corresponding antidote can rescue other poison alleles. Column 9 notes the AmFRET values are comparable to the wild-type Wtf4poison-mEos or monomer-mEos. Finally, column 10 notes the figures/supplemental figures where these alleles can be found. Rows are separated based on the wild-type allele that the mutants were constructed from, where applicable. ND indicates that the specified experiment was not done. (XLSX)