Metabolomics analysis of the midgut of Dredd mutant Drosophila, with or without PGRP-LE overexpression in enterocytes

We sought to determine whether the pattern recognition receptor PGRP-LE acts independently of Relish/NF-kB signaling to alter metabolism in the midgut (the midgut is the equivalent of the small intestine in Drosophila). To test this possibility, we performed metabolomics analyses in Dredd mutant midguts (“Dredd_mex_plus”) and Dredd mutant midguts with PGRP-LE overexpression in enterocytes (“Dredd_mex_LE”). We used a Dredd mutant background because this mutation blocks the induction of Relish/NF-kB downstream of PGRP-LE. We have used germ-free, 7-day-old males for these analyses. The following stocks were used to generate these genotypes: Dredd[D44] (RRID:BDSC_ 80924), mex-GAL4 (RRID:BDSC_ 91368) and UAS-PGRP-LE (RRID:BDSC_33054). The dataset contains the liquid chromatography mass spectrometry (LC-MS) raw files (2 zipped files containing the Thermo .raw files from the IC-MS (anions) and the Bz-UHPLC-MS (amines) measurements). The dataset also contains two tables with the peak information of the steady state metabolite measurements (Compound name, expected RT (retention time, min), Filename, Sample type (sample, method blank (MB), quality control (pool) and reference compound mixture (Std Mix)), Injection Volume (uL), sample type, peak height (AU), peak area (AU), actual RT (min), RT Delta, formula, adduct, m/z (Expected), m/z (Apex), m/z (Delta in ppm) and S/N (signal-to-noise)). Data were obtained using either anion chromatography coupled to high resolution mass spectrometry (for anionic compounds, including intermediates of the TCA cycle, glycolysis, pentose phosphate pathway and nucleotides) or ultra-high performance liquid chromatography (UHPLC) coupled to high resolution mass spectrometry (for amine-containing compounds, including amino acids and polyamines).