Table2.XLSX

HIGHLIGHTSMetabolites and transcripts related to plant physiology in salt stress conditions, especially to the recovery process were disclosed in peanut.Peanut (Arachis hypogaea L.) is considered as a moderately salt-sensitive species and thus soil salinity can be a limiting factor for peanut cultivation. To gain insights into peanut plant physiology in response to salt stress and alleviation, we comprehensively characterized leaf relative electrolyte leakage (REC), photosynthesis, leaf transpiration, and metabolism of plants under salt stress and plants that were subjected to salt stress followed by salt alleviation period. As expected, we found that REC levels were higher when plants were subjected to salt stress compared with the untreated plants. However, in contrast to expectations, REC was even higher compared with salt treated plants when plants were transferred from salt stress to standard conditions. To decipher REC variation in response to salt stress, especial during the recovery, metabolite, and transcript variations were analyzed by GC/MS and RNA-seq method, respectively. Ninety two metabolites, among total 391 metabolites identified, varied in response to salt and 42 metabolites responded to recovery specially. Transcriptomics data showed 1,742 in shoots and 3,281 in roots transcript varied in response to salt stress and 372 in shoots and 1,386 transcripts in roots responded specifically to recovery, but not salt stress. Finally, 95 transcripts and 1 metabolite are indicated as candidates involved in REC, photosynthesis, transpiration, and Na+ accumulation variation were revealed by using the principal component analysis (PCA) and correlation analysis. This study provides valuable information on peanut response to salt stress and recovery and may inspire further study to improve salt tolerance in peanut germplasm innovation.

本研究揭示了与植物生理学相关的代谢物和转录本，特别是花生在盐胁迫条件下的恢复过程。花生（Arachis hypogaea L.）被认为是一种对盐分中度敏感的物种，因此土壤盐分可能成为花生栽培的限制因素。为了深入了解花生植物在盐胁迫及缓解过程中的生理学反应，我们对花生叶片相对电解质渗漏（REC）、光合作用、叶片蒸腾作用以及在盐胁迫及盐分缓解期后的植物代谢进行了全面表征。正如预期，与未处理植物相比，遭受盐胁迫的植物REC水平更高。然而，与预期相反，当植物从盐胁迫转移到标准条件时，REC甚至比盐处理植物更高。为了解析盐胁迫下REC的变化，特别是在恢复期，我们分别通过GC/MS和RNA-seq方法分析了代谢物和转录本的变化。在总共识别的391种代谢物中，有92种代谢物对盐分反应，其中42种代谢物对恢复过程有特殊反应。转录组学数据显示，在茎中检测到1,742个、在根中检测到3,281个转录本对盐胁迫有反应，而在茎中检测到372个、在根中检测到1,386个转录本对恢复有特异性反应，而非盐胁迫。最终，通过主成分分析（PCA）和相关性分析，揭示了95个转录本和1种代谢物是参与REC、光合作用、蒸腾作用和Na+积累变化的候选因子。本研究为花生对盐胁迫和恢复的响应提供了宝贵信息，可能激发进一步研究以提升花生种质资源抗盐性的创新。